Isolation, Purification and Characterization of the Active Compounds from Ganoderma spp. and Evaluation of its Activities in Inhibiting the Growth of the Pathogenic fungi Fusarium oxysporum and Alternaria tenuissima

Abstract The main objectives of the present study include purification active Compounds from medecinal mushroom, Ganoderma spp. for inhibiting growth pathogenic fungi Fusarium oxysporum and Alternaria tenuissima . antifungal activity protein content separated by gel filtration chromatography four strains mushroom; G. resinaceum strain Has.AA-8(ON834527.1), applanatum Has.AA-6 (ON834523.1), 1049 (MN448375) lucidum Has.AA-7 (ON834541.1)was studied. results showed presence 6,8,8 9 inhibitory peaks against phytopathogenic fungi, related to these mushroom strains, respectively. Among fractions, three enzymes namely, chitinase, β-glucanase protease were identified. highest chitinase effect recorded in fractions 14 16 contents 1049, which, reached 3.11 3.06 units / ml, respectively, while inhibition zones 1.4 cm both 0.9 1 F. A. , activities 1.62 unit/ml which record zone 1.3 compared (1.4 cm) also 28.04 27.75 32 46 Has.AA-8, with (1.7 (1.2 1.1 Its clearly this results, produces a wide range substances that inhibit tenuissima.